海藻种质库研究成果—裙带菜基础和应用研究

海藻种质库研究团队长期致力于裙带菜基础和应用研究,取得系列重要进展,包括:(1)培育了我国目前仅有的两个裙带菜国家新品种“海宝1、2号”;(2)利用SNP标记技术构建了首张高密度遗传连锁图谱[1];(3)完成了全长转录组测序,结合二代测序技术分析了孢子叶发育过程中基因表达规律[2];(4)完成了入侵德国北部的裙带菜群体的溯源[3];(5)利用PacBio三代测序平台和Hi-C技术,在国际上首次完成了全基因组在染色体水平组装和注释[4];(6)国际上首次发现雌雄同体配子体的存在[5];(7)成功建立了单性游孢子杂交技术[6]

裙带菜原产于西北太平洋沿海,是中日韩三国重要经济褐藻。近年来,裙带菜作为一种重要的入侵性海藻引起西方学者的广泛关注。上述研究成果为裙带菜遗传育种和入侵机制的研究奠定了基础。

 

Reproduction biology and farming research of Undaria pinnatifida

 

The research team of the Marine Algal Culture Collection Centre has devoted long-term efforts to fundamental and applied research of Undaria pinnatifida. The team has made a series of important progresses in the last decade, including: (1) has bred the only two elite national varieties “Haibao No 1 and 2” certificated by the Ministry of Agriculture; (2) has constructed the first high-density genetic linkage map based on SNP markers[1]; (3) has sequenced the full-length transcriptome and analyzing the transcriptomic changes during the development of the sporophyll[2];(4) has tracked the source of the newly invaded populations in the north sea of Germany[3]; (5)has obtained for the first time the whole genome sequence and finished the chromosome-level assembly and annotation[4];(6) has observed and reported the zoospore-derived hermaphroditic gametophyte for the first time[5]; (7) has established male and female zoospore hybrid breeding technology[6].

As an important economic kelp species native to the Northwest Pacific, U.pinnatifida has been farmed for a long history in China, Japan and Korea. It has drawn increasing concern due to its worldwide spread as an alien species in recent years. The above-mentioned achievements have laid foundations for future investigations on the genetic breeding and the invasion mechanisms of this important seaweed.

[1] Shan T, Pang S, Li J, Li X, Su L. 2015. Construction of a high-density genetic map and mapping of a sex-linked locus for the brown alga Undaria pinnatifida (Phaeophyceae) based on large scale marker development by specific length amplified fragment (SLAF) sequencing. Bmc Genomics 16.

https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-015-2184-y

[2] Shan T, Li Q, Wang X, Pang S. 2020a. Full-length transcriptome sequencing and comparative transcriptomic analysis of different developmental stages of the sporophyll in Undaria pinnatifida (Laminariales: Alariaceae). Journal of Applied Phycology: 1-12.

https://link.springer.xilesou.top/article/10.1007/s10811-020-02080-w

[3] Shan T, Pang S, Wang X, Li J, Su L, Schiller J, Lackschewitz D, Hall-Spencer JM, Bischof K. 2019. Genetic analysis of a recently established Undaria pinnatifida (Laminariales: Alariaceae) population in the northern Wadden Sea reveals close proximity between drifting thalli and the attached population. European Journal of Phycology 54: 154-161.

https://www.tandfonline.xilesou.top/doi/abs/10.1080/09670262.2018.1532116

[4] Shan T, Yuan J, Su L, Li J, Leng X, Zhang Y, Gao H, Pang S. 2020b. First Genome of the Brown Alga Undaria pinnatifida: Chromosome-Level Assembly Using PacBio and Hi-C Technologies. Frontiers in Genetics 11: 140.

https://www.frontiersin.org/articles/10.3389/fgene.2020.00140/full

[5] Li J, Pang S, Shan T, Liu F, Gao S. 2014. Zoospore-derived monoecious gametophytes in Undaria pinnatifida (Phaeophyceae). Chinese Journal of Oceanology and Limnology 32: 365-371.

https://link.springer.xilesou.top/article/10.1007/s00343-014-3139-x

[6] Li J, Pang S, Shan T. 2017. Existence of an intact male life cycle offers a novel way in pure-line crossbreeding in the brown alga Undaria pinnatifida. Journal of Applied Phycology 29: 993-999.

https://link.springer.xilesou.top/article/10.1007/s10811-016-1005-2

 

 

Leave a Reply

Your email address will not be published. Required fields are marked *